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collagen type i alpha 1 col1a1  (R&D Systems)


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    R&D Systems collagen type i alpha 1 col1a1
    Collagen Type I Alpha 1 Col1a1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+type+i+alpha+1+col1a1/Recombinant+Human+Pro-Collagen+I+alpha+1%2FCOL1A1+Protein%2C+CF/10__1002_slash_eom2__70061-147-19-47
    Average 94 stars, based on 14 article reviews
    collagen type i alpha 1 col1a1 - by Bioz Stars, 2026-09
    94/100 stars

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    Concentration Assay:

    Article Title: Platelet-Released Growth Factors Induce Genes Involved in Extracellular Matrix Formation in Human Fibroblasts
    Article Snippet: .. The concentration of fibronectin 1 (FN1) and collagen type I alpha 1 (COL1A1) in the supernatants of PRGF-treated fibroblasts were determined by ELISA (R&D Systems, Minneapolis, MN; catalog no. DY1918-05 and DY6220-05). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Platelet-Released Growth Factors Induce Genes Involved in Extracellular Matrix Formation in Human Fibroblasts
    Article Snippet: .. The concentration of fibronectin 1 (FN1) and collagen type I alpha 1 (COL1A1) in the supernatants of PRGF-treated fibroblasts were determined by ELISA (R&D Systems, Minneapolis, MN; catalog no. DY1918-05 and DY6220-05). ..

    Article Title: Platelet-Released Growth Factors and Platelet-Rich Fibrin Induce Expression of Factors Involved in Extracellular Matrix Organization in Human Keratinocytes
    Article Snippet: .. The supernatants of PRGF-stimulated keratinocytes were analyzed by ELISA specific for fibronectin 1, (FN1), matrix metalloproteinase-9 (MMP9) and collagen type I alpha 1 (COL1A1) using DuoSet ELISA kits from R&D systems (Minneapolis, MN, USA) according to the manufacturers’ instructions. ..

    Article Title: Biodegradable, Breathable, and Skin‐Friendly Solution‐Blown Quercetin Nanofibers for Antiviral Nonwoven Filter
    Article Snippet: Subsequently, cells were treated with (3 and 10) μM of QU for 24 h. After that, the cells were exposed to 20 ng/mL TNF–α (PeproTech). .. Following that, the medium was collected from cells, and the secretion levels of matrix metalloproteinase- 1 (MMP- 1) and collagen type I alpha 1 (COL1A1) were measured using a Human Total MMP- 1 DuoSet ELISA kit and a Human Pro- Collagen I alpha 1 DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), respectively. .. The absorbance was determined at 550/600 nm by a microplate reader SPARK 10 M (Molecular Devices).



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    Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 <t>(Col1A1)</t> ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).
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    collagen type i alpha 1 col1a1  (R&D Systems)
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    Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 <t>(Col1A1)</t> ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).
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    Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 <t>(Col1A1)</t> ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).
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    Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 <t>(Col1A1)</t> ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).
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    Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 <t>(Col1A1)</t> ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).
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    The antioxidant peptides suppressed UVB-induced depletion of <t>collagen</t> <t>type</t> <t>I</t> precursor <t>(COL1A1).</t> (A) Representative immunofluorescence images of COL1A1 photographed by confocal microscopy. COL1A1 was stained with Alexa Fluor 594-conjugated antibody (red), and nuclei were stained with DAPI (blue). Scale bar = 20 μm. Quantitation is shown on the right (B) . (*** P < 0.001, **** P < 0.0001, n = 4).
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    The antioxidant peptides suppressed UVB-induced depletion of <t>collagen</t> <t>type</t> <t>I</t> precursor <t>(COL1A1).</t> (A) Representative immunofluorescence images of COL1A1 photographed by confocal microscopy. COL1A1 was stained with Alexa Fluor 594-conjugated antibody (red), and nuclei were stained with DAPI (blue). Scale bar = 20 μm. Quantitation is shown on the right (B) . (*** P < 0.001, **** P < 0.0001, n = 4).
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    collagen type i alpha 1 chain col1a1  (Proteintech)
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    The antioxidant peptides suppressed UVB-induced depletion of <t>collagen</t> <t>type</t> <t>I</t> precursor <t>(COL1A1).</t> (A) Representative immunofluorescence images of COL1A1 photographed by confocal microscopy. COL1A1 was stained with Alexa Fluor 594-conjugated antibody (red), and nuclei were stained with DAPI (blue). Scale bar = 20 μm. Quantitation is shown on the right (B) . (*** P < 0.001, **** P < 0.0001, n = 4).
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    The antioxidant peptides suppressed UVB-induced depletion of <t>collagen</t> <t>type</t> <t>I</t> precursor <t>(COL1A1).</t> (A) Representative immunofluorescence images of COL1A1 photographed by confocal microscopy. COL1A1 was stained with Alexa Fluor 594-conjugated antibody (red), and nuclei were stained with DAPI (blue). Scale bar = 20 μm. Quantitation is shown on the right (B) . (*** P < 0.001, **** P < 0.0001, n = 4).
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    Image Search Results


    Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 (Col1A1) ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).

    Journal: Scientific Reports

    Article Title: C57BL/6J mice best recapitulate fibrosis and inflammatory pathophysiology in syngeneic mouse model of endometriosis

    doi: 10.1038/s41598-025-13900-9

    Figure Lengend Snippet: Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 (Col1A1) ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).

    Article Snippet: The levels of Collagen Type I (COL1A1) in ectopic tissue lysates were quantified using a commercially available ELISA kit (Krishgen Biosystems, USA, #111111111).

    Techniques: Staining, Enzyme-linked Immunosorbent Assay

    The antioxidant peptides suppressed UVB-induced depletion of collagen type I precursor (COL1A1). (A) Representative immunofluorescence images of COL1A1 photographed by confocal microscopy. COL1A1 was stained with Alexa Fluor 594-conjugated antibody (red), and nuclei were stained with DAPI (blue). Scale bar = 20 μm. Quantitation is shown on the right (B) . (*** P < 0.001, **** P < 0.0001, n = 4).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Antiphotoaging effects of a group of antioxidant peptides through downregulating matrix metalloproteinases and inflammation factors

    doi: 10.3389/fcell.2025.1649391

    Figure Lengend Snippet: The antioxidant peptides suppressed UVB-induced depletion of collagen type I precursor (COL1A1). (A) Representative immunofluorescence images of COL1A1 photographed by confocal microscopy. COL1A1 was stained with Alexa Fluor 594-conjugated antibody (red), and nuclei were stained with DAPI (blue). Scale bar = 20 μm. Quantitation is shown on the right (B) . (*** P < 0.001, **** P < 0.0001, n = 4).

    Article Snippet: Western blotting was performed to detect the protein expression of β-actin, MMP1, MMP2, MMP3, MMP9, JNK, p-JNK, p38, p-p38, IL-6, and IL-1β in HDF-a cells and mouse skin (β-actin, MMP2, MMP9, p38, phosphorylated p38 (p-p38), JNK, phosphorylated JNK (p-JNK) and alpha-1 type I collagen (COL1A1) were purchased from Cell Signaling Technology, Inc. (Boston, United States); MMP1 and MMP3 were acquired from Proteintech Group, Inc. (Wuhan, China)).

    Techniques: Immunofluorescence, Confocal Microscopy, Staining, Quantitation Assay