collagen type i alpha 1 col1a1  (R&D Systems)

Journal: Regenerative Biomaterials

Article Title: Iron–quercetin nanocomplex preconditioning reprograms the mesenchymal stem cell secretome to drive angiogenic, fibroblast and immunoregulatory wound repair

doi: 10.1093/rb/rbag132

Figure Lengend Snippet: Secretome derived from IronQ-preconditioned ADSCs enhances fibroblast proliferation and activation-associated responses. ( A ) Proliferation of dermal fibroblasts following exposure to conditioned medium from IronQ-preconditioned ADSCs (IronQ-CM), evaluated using the CCK-8 assay at days 1, 3, 5, and 7. Quantitative results are expressed as mean ± standard deviation (SD) from three independent experiments. * p < 0.05 versus corresponding control groups. ( B ) Collagen type I (Col-I) levels in fibroblast culture supernatants following treatment with ADSC-derived conditioned media, determined by ELISA. Data are shown as mean ± SD from three independent experiments. ( C–E ) Relative mRNA expression of fibroblast activation-associated genes, including COL1A1 ( C ), ACTA2 (encoding α-smooth muscle actin; α-SMA) ( D ), and fibronectin FN1 ( E ) determined by quantitative real-time PCR (qRT-PCR). Gene expression levels were normalized to GAPDH and calculated using the comparative 2 −ΔΔ Ct method. Results represent mean ± SD from three independent experiments.

Article Snippet: Collagen type I secretion in HDFs exposed to ADSC-derived secretomes was quantified using a human collagen type I alpha 1 (COL1A1) ELISA kit (ABclonal, Wuhan, China).

Techniques: Derivative Assay, Activation Assay, CCK-8 Assay, Standard Deviation, Control, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: Antiphotoaging effects of a group of antioxidant peptides through downregulating matrix metalloproteinases and inflammation factors

doi: 10.3389/fcell.2025.1649391

Figure Lengend Snippet: The antioxidant peptides suppressed UVB-induced depletion of collagen type I precursor (COL1A1). (A) Representative immunofluorescence images of COL1A1 photographed by confocal microscopy. COL1A1 was stained with Alexa Fluor 594-conjugated antibody (red), and nuclei were stained with DAPI (blue). Scale bar = 20 μm. Quantitation is shown on the right (B) . (*** P < 0.001, **** P < 0.0001, n = 4).

Article Snippet: Western blotting was performed to detect the protein expression of β-actin, MMP1, MMP2, MMP3, MMP9, JNK, p-JNK, p38, p-p38, IL-6, and IL-1β in HDF-a cells and mouse skin (β-actin, MMP2, MMP9, p38, phosphorylated p38 (p-p38), JNK, phosphorylated JNK (p-JNK) and alpha-1 type I collagen (COL1A1) were purchased from Cell Signaling Technology, Inc. (Boston, United States); MMP1 and MMP3 were acquired from Proteintech Group, Inc. (Wuhan, China)).

Techniques: Immunofluorescence, Confocal Microscopy, Staining, Quantitation Assay